Effect of Reactive Oxygen Species (ROS) on the Invasion of Listeria monocytogenes to 7721 Epithelial Cells

doi:10.7506/spkx1002-6630-201521023

Abstract

Abstract:

Diphenyleneiodonium chloride (DPI), which can inhibit the activity of NADPH oxidase and reduce endogenous
reactive oxygen species (ROS), was used to reduce the generation of ROS in Listeria monocytogenes, which was then
allowed to invade 7721 epithelial cells of human hepatocellular carcinoma. The results showed that the endogenous ROS
in L. monocytogenes was markedly decreased in the presence of DPI in a concentration-dependent way. Within the DPI
concentration range of 1–10 μmol/L, as the level of ROS in reduced, its ability to invade 7721 epithelial cells increased.
This illustrates that L. monocytogenes may have a NADPH oxidase similar to that of higher plants and animals, which is
responsible for the production of ROS and regulates the invasion ability possibly by mediating ROS production, suggesting
that NADPH oxidase may be an important signal molecule for L. monocytogenes to invade 7721 epithelial cells.

Key words: Listeria monocytogenes, reactive oxygen species (ROS), invasion, epithelial cells

CLC Number:

Q932

CHEN Guowei, WU Man, ZHANG Chao, LIU Wukang, DING Chengchao, WU Shuyan, LI Sen, LIU Qing. Effect of Reactive Oxygen Species (ROS) on the Invasion of Listeria monocytogenes to 7721 Epithelial Cells[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201521023.

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Effect of Reactive Oxygen Species (ROS) on the Invasion of Listeria monocytogenes to 7721 Epithelial Cells

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