Determination of Free Phytosterols in Litchi Pulp by HPLC-DAD Coupled with Second-Order Calibration Based on Alternating Trilinear Decomposition

doi:10.7506/spkx1002-6630-201524031

Abstract

Abstract:

A rapid method to quantify free phytosterols in litchi pulp was established with high performance liquid
chromatography with diode array detection (HPLC-DAD) coupled with second-order calibration method based on
alternating trilinear decomposition (ATLD) algorithm. The chromatographic conditions were simplified by applying secondorder
calibration method. The mobile phase was 95% aqueous acetonitrile (V/V) at a flow rate of 1.0 mL/min. The injection
volume was 20.0 μL. The temperature of inertsil ODS-SP (150 mm × 4.6 mm, 5 μm) column was set at 30 ℃. The detection
wavelengths were 190–340 nm. The average spiked recoveries of campesterol and stigmasterol in litchi pulp were (95.25 ±
0.78)% and (96.83 ± 1.01)%, respectively. The pulp of “Lanzhu” and “Wuye” litchi was detected to contain 24.1 and 27.4 μg/g
campesterol, and 25.8 and 26.7 μg/g stigmasterol, respectively. The newly established method is simple, accurate, highly
sensitive and reliable, and can be used to determine free phytosterols in litchi pulp.

Key words: high performance liquid chromatography (HPLC), diode array detector (DAD), alternating trilinear decomposition (ATLD), second-order calibration, phytosterols

CLC Number:

TS207.3

XIANG Leiwen1, CHEN Guotai1, WENG Jiamin1, CHEN Wentao1, WANG Shaoyun2,*. Determination of Free Phytosterols in Litchi Pulp by HPLC-DAD Coupled with Second-Order Calibration Based on Alternating Trilinear Decomposition[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201524031.

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Determination of Free Phytosterols in Litchi Pulp by HPLC-DAD Coupled with Second-Order Calibration Based on Alternating Trilinear Decomposition

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