DiversiLab Typing of Listeria monocytogenes Isolated from Poultry Products

doi:10.7506/spkx1002-6630-201524041

Abstract

Abstract:

To study the homologous relationship of Listeria monocytogenes strains isolated from poultry products, which
can provide a scientific basis for forecasting and early warning of food security and provide technical support for preventing
food-borne diseases caused by L. monocytogenes. Methods: A total of 25 strains of L. monocytogenes isolated from the
poultry products in the Fujian Entry-Exit Inspection and Quarantine Bureau from 2001 to 2010 were typed by DiversiLab
typing system through DNA extraction, repetitive sequence-based polymerase chain reaction (rep-PCR), and microfluidic
chip. Results: At the similarity coefficient of 95% and 97%, 25 strains were accordingly divided into 6 groups (G) and
11 patterns (P), respectively. Conclusions: The genetic relatedness among the 25 strains could be accurately revealed by
DiversiLab typing. Automated DiversiLab typing system is a fast, easily operated, highly discriminatory and efficient genetyping
method. It can be recommended as an epidemiological analysis tool for foodborne illness.

Key words: DiversiLab typing system, Listeria monocytogenes, rep-PCR, epidemiological analysis

CLC Number:

Q936

ZHENG Jing1, TANG Zhongwei1,2, CHEN Bin1,HUANG Xiaorong1, LIN Jie1,ZHANG Tiyin1. DiversiLab Typing of Listeria monocytogenes Isolated from Poultry Products[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201524041.

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DiversiLab Typing of Listeria monocytogenes Isolated from Poultry Products

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