Abstract:

Objective: To observe the protective effect of fibrinolytic enzyme from Lyophyllum ulmarium (L.u) on H2O2-
induced oxidative damage of human umbilical vein endothelial cells (HUVECs). Methods: H2O2 was used to induce the
oxidative injury model of HUVECs. The survival rate of HUVECs was determined by MTT assay. The mitochondrial
transmembrane potential (ΔΨm) and reactive oxygen species (ROS) of HUVECs were detected by flow cytometry. The
expression of Caspase-8, Caspase-9 and Caspase-3 proteins was detected by Western blotting. Results: L.u could increase the
survival rate of endothelial cells, inhibit intracellular ROS production and mitochondrial transmembrane potential reduction
in HUVECs, and down-regulate the expression of Caspase-8, Caspase-9 and Caspase-3 proteins. Conclusion: The fibrinolytic
enzyme from Lyophyllum ulmarium has protective effect on vascular endothelial cells due to the inhibition of apoptosis.

Key words: Lyophyllum ulmarium, fibrinolytic enzyme, human umbilical vein endothelial cell, mitochondrial transmembrane potential, reactive oxygen species, apoptosis