Determination of Scopoletin in Noni Juice and Traditional Chinese Medicinal Compound Health Product Containing Noni Juice by HPLC

doi:10.7506/spkx1002-6630-201604015

Abstract

Abstract:

A high performance liquid chromatography (HPLC) method was established for the determination of scopoletin
in noni juice and a traditional Chinese medicinal compound health product containing noni juice. The chromatographic
separation was performed on an Elite SinoChrom ODS-BP column (4.6 mm × 200 mm, 5 μm) using acetonitrile-0.1%
phosphoric acid aqueous solution (30:70, V/V) as the mobile phase at a flow rate of 1 mL/min. The column temperature
was 25 ℃, and the detection wavelength was set at 348 nm. The results showed a good linear relationship between peak
area and scopoletin concentration in the range of 0.5–20 μg/mL. The average recoveries for noni juice and the health
product containing noni juice were 104.05% and 97.11%, with relative standard deviations (RSDs) of 0.81% and 1.99%,
respectively. This method proved to be accurate and reliable and could be used to qualitatively and quantitatively determine
scopoletin in noni juice and the compound health product containing noni juice.

Key words: noni juice, health product, scopoletin, high performance liquid chromatography (HPLC)

CLC Number:

R284.2

CHEN Chen, LIU Pinghuai, LUO Ning, LI Ang, HE Yifei, FU Jing. Determination of Scopoletin in Noni Juice and Traditional Chinese Medicinal Compound Health Product Containing Noni Juice by HPLC[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201604015.

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Determination of Scopoletin in Noni Juice and Traditional Chinese Medicinal Compound Health Product Containing Noni Juice by HPLC

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