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Protective Effect of Individual and Combined Selenium and VE on H2O2-Induced Oxidative Damage in HUVECs

LI Xia, YAO Zhao, ZHANG Yunlong, LI Hongyan*   

  1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
  • Online:2016-04-15 Published:2016-04-13

Abstract:

Objective: To study the protective effect of individual and combined selenium and VE on human umbilical vein
endothelial cell (HUVECs) with H2O2-induced oxidative damage. Methods: HUVECs cultured in vitro were divided into
normal control group, model control group and five treatment groups including L-Se-methylselenoeysteine (L-SeMSC)
group, sodium selenite (SS) group, VE group, L-SeMSC + VE group, and SS + VE group. The HUVECs were injured by
H2O2 after they were incubated for 24 h. The effects of all these treatments on cell viability were assayed by MTT. The
activity of antioxidant enzymes (SOD and GSH-Px) and the content of MDA were determined. Results: Compared with
the model group, treatment with 0.1 mg/L SS, or 10 mg/L VE could increase the viability of HUVECs, reduce the content
of MDA, and increase the activity of antioxidant enzymes (SOD and GSH-Px). The combination of SS and VE exerted a
synergistic antioxidant effect at the cellular level, but L-SeMSC did not exhibit a significant difference when compared with
the control group. Conclusion: both SS and VE possess protective effect on H2O2-induced damage in HUVECs, and can
enhance cell antioxidant activity in a synergistic manner.

Key words: selenium, VE, human umbilical vein endothelial cells (HUVECs), synergistic antioxidant activity

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