Abstract:

Objective: To investigate the proliferative and protective effects of γ-oryzanol on L02 cells. Methods: The model
of oxidative damage was established on L02 cells induced by H2O2. Cell viability, the contents of malondialdehyde (MDA)
and glutathione (GSH), the activities of superoxide dismutase (SOD) and catalase (CAT), and the level of ROS were
assayed on L02 cells treated with γ-oryzanol at various concentrations in vitro. Results: The cell viability was enhanced
significantly, and the levels of ROS and MDA were decreased significantly, whereas the content of GSH and the activities of
CAT and SOD were significantly increased by 0.1–0.4 mmol/L γ-oryzanol treatment after H2O2-induced oxidative damage
in a dose dependent manner. Conclusion: γ-Oryzanol can effectively attenuate H2O2-induced oxidative damage at a dose of
0.1–0.4 mmol/L and maintain the normal physiology of the cells.

Key words: γ-oryzanol, oxidative damage, L02 cell