Optimization of Foam Separation of Dioscin from Dioscorea zingiberensis C. H. Wright by Response Surface Methodology

doi:10.7506/spkx1002-6630-201608005

Abstract

Abstract:

A novel method for recovering dioscin produced from the rhizomes of Dioscorea zingiberensis C. H. Wright by
enzymatic hydrolysis and ultrasonic extraction with aqueous ethanol as the solvent was established by foam separation in this
study. The effect of dilution factor, temperature and filling volume in the separation column on the recovery and enrichment
ratio of dioscin was addressed using combination of one-factor-at-a-time method and response surface methodology. A
dilution factor of 84, an operating temperature of 20 ℃ and filling 436 mL of sample into the separation column provided
optimal conditions for separation of dioscin. Under these conditions, the maximum enrichment ratio and recovery of
dioscin of 7.53 and 91.08% respectively were obtained. The procedure presented in this study is an effective, simple
and environmentally compatible method thathas a great potential to be industrialized to separate dioscin from Dioscorea
zingiberensis C.H. Wright using foam separation.

Key words: Dioscorea zingiberensis C. H. Wright, dioscin, foam separation, response surface methodology

CLC Number:

O629.9

GAO Zhongchao, ZHANG Wei, CHEN Yuantao, LIU Haibin, LIU Long, LEI Lei. Optimization of Foam Separation of Dioscin from Dioscorea zingiberensis C. H. Wright by Response Surface Methodology[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201608005.

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Optimization of Foam Separation of Dioscin from Dioscorea zingiberensis C. H. Wright by Response Surface Methodology

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