Cryoprotective Effect and Mechanism of Dextran with Different Molecular Weights on Denaturation of Myofibrillar Protein from Grass Carp (Ctenopharyngodon idella)

doi:10.7506/spkx1002-6630-201610049

Abstract

Abstract:

The present study investigated the cryoprotective effect of dextran with different molecular weights on
denaturation of myofibrillar protein from grass carp (Ctenopharyngodon idella). The content of salt soluble protein,
Ca2+-ATPase activity, surface hydrophobicity, total sulfhydryl group and active sulfhydryl group were evaluated. Results
showed that the denaturation of grass carp myofibrillar protein was significantly delayed by dextran during frozen storage,
and the effect of dextran (0.5%) was better than that of commercial antifreeze (4% sucrose + 4% D-sorbitol). Low molecular
weight dextran (T7) was better than high molecular weight dextran (T20) in protecting the Ca2+-ATPase activity and active
sulfhydryl group and also in delaying the increase of surface hydrophobicity and the decrease of sulfhydryl group after
15 d frozen storage. Thus, the low molecular weight dextran is recommended to be used in the fishery industry as a
promising cryoprotectant.

Key words: grass carp, myofibrillar protein, dextran, molecular weight, frozen denaturation

CLC Number:

TS254.4

LU Yaobin, XIONG Guangquan, LI Xin, WU Wenjin, QIAO Yu, DING Anzi, LIAO Li, WANG Jun, WANG Lan. Cryoprotective Effect and Mechanism of Dextran with Different Molecular Weights on Denaturation of Myofibrillar Protein from Grass Carp (Ctenopharyngodon idella)[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201610049.

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Cryoprotective Effect and Mechanism of Dextran with Different Molecular Weights on Denaturation of Myofibrillar Protein from Grass Carp (Ctenopharyngodon idella)

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