Cultivar Selection for Isoflavone Accumulation and Kinetics of Soybean Germination under UV-B Radiation

doi:10.7506/spkx1002-6630-201613019

Abstract

Abstract:

Soybean cultivars Tian Jiao (TJ), Su Qing1 (SQ1), Su Qing3 (SQ3) and 95You1 (95Y1) were germinated
for 5 days under UV-B radiation to select the best cultivar for isoflavone accumulation based on the content of
isoflavone and the activity of key enzymes and comprehensive analysis of physio-biochemical indexes. Results
showed that sprouts of the TJ cultivar had the highest levels of isoflavone content, phenylalnine ammonialyase
(PAL) activity, and chalcone synthase (CHS) activity as well as the best quality. Meanwhile, the germination
percentage, sprout length, respiratory rate, and the contents of free amino acids and reduced sugar of the cultivar
TJ were higher than those of other tested cultivars. Thus, the cultivar TJ could be considered as a good source
for isoflavone accumulation. The content of isoflavone significantly increased with germination time, which was
3.02 times higher at 7 d than at 0 d. The activity of PAL likewise increased while the activity of CHS first increased and then
decreased. Macromolecular compounds such as soluble protein trended to decrease whereas small molecules such as free
amino acids increased gradually. In conclusion, TJ was screened out for isoflavone accumulation under UV-B radiation.

Key words: soybean, germination, isoflavone, UV-B radiation, accumulation

CLC Number:

TS214.2

CHEN Xu, YANG Runqiang, GU Zhenxin. Cultivar Selection for Isoflavone Accumulation and Kinetics of Soybean Germination under UV-B Radiation[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201613019.

References

[1] 陈振, 康玉凡. 豆类种子及萌发过程中功效性成分研究概

述[J]. 中国食物与营养, 2012, 18(10): 27-32. DOI:10.3969/

j.issn.1006-9577.2012.10.008.

[2] 练云, 梁慧珍, 余永亮, 等. 异黄酮生物合成途径研究进展[J].

安徽农业科学, 2012, 40(23): 11543-11544. DOI:10.3969/

j.issn.0517-6611.2012.23.003.

[3] WATANABE S, YAMAGUCHI M, SOBUE T, et al. Pharmacokinetics

of soybean isoflavones in plasma, urine and feces of men after

ingestion of 60 g baked soybean powder (kinako)[J]. Journal of

Nutrition, 1998, 128(10): 1710-1715.

[4] 李振艳, 张永忠, 任红波, 等. 大豆发芽过程中异黄酮、γ-氨基丁酸

等成分含量变化的研究[J]. 食品工业科技, 2009, 30(12): 356-358;

361. DOI:10.13386/j.issn1002-0306.2009.12.066.

[5] 来吉祥, 魏少敏, 方云, 等. 谷豆萌芽过程中成分变化、生物活性及

应用研究进展[J]. 食品与生物技术学报, 2013, 32(10): 1016-1024.

[6] 欧阳光察, 薛应龙. 植物苯丙烷类代谢的生理意义及其调控[J].

植物生理学通讯, 1988, 24(3): 9-16. DOI:10.13592/j.cnki.

ppj.1988.03.003.

[7] DIXON R A, PAIVS N L. Stress-induced phenylpropanoid

metabolism[J]. Plant Cell, 1995, 7(7): 1085. DOI:10.1105/tpc.7.7.1085.

[8] 谢灵玲, 赵武玲. 光照对大豆叶片苯丙氨酸裂解酶(PAL)基因表

达及异黄酮合成的调节[J]. 植物学通报, 2000, 17(5): 443-449.

DOI:10.3969/j.issn.1674-3466.2000.05.011.

[9] KIM H K, JANG Y H, BAEK I S, et al. Polymorphism and expression

of isoflavone synthase genes from soybean cultivars[J]. Molecules &

Cells, 2005, 19(1): 67-73.

[10] LUCZKIEWICZ M, GLOD D. Callus cultures of Genista plants:

in vitro material producing high amounts of isoflavones of

phytoestrogenic activity[J]. Plant Science, 2003, 165(5): 1101-1108.

DOI:10.1016/S0168-9452(03)00305-4.

[11] 李菊艳, 姚文秋, 宫绍斌, 等. 环境因素对大豆异黄酮的影响研究进

展[J]. 中国农学通报, 2010, 26(9): 167-170.

[12] KOSSLAK R M, BOOKLAND R, BARKEI J, et al. Induction of

Bradyrhizobium japonicum common nod genes by isoflavones isolated

from Glycine max[J]. Proceedings of the National Academy of

Sciences, 1987, 84(21): 7428-7432. DOI:10.1073/pnas.84.21.7428.

[13] RYDER T B, CRAMER C L, BELL J N, et al. Elicitor rapidly induces

chalcone synthase mRNA in Phaseolus vulgaris cells at the onset

of the phytoalexin defense response[J]. Proceedings of the National

Academy of Sciences, 1984, 81(18): 5724-5728. DOI:10.1073/

pnas.81.18.5724.

[14] 沈黎明, 孙君明. 不同光照条件下大豆体内异黄酮的含量与

分布[J]. 中国油料作物学报, 1999, 21(2): 36-40. DOI:10.3321/

j.issn:1007-9084.1999.02.011.

[15] WANG H J, MURPHY P A. Isoflavone composition of American

and Japanese soybeans in Iowa: effects of variety, crop year, and

location[J]. Journal of Agricultural and Food Chemistry, 1994, 42(8):

1674-1677. DOI:10.1021/jf00044a017.

[16] 李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社,

2000: 61-201.

[17] 何恩铭, 沈瑞池, 王伟, 等. 紫外分光光度法测定豆渣提取物中大豆

异黄酮含量的研究[J]. 大豆科学, 2013, 32(6): 818-820.

[18] SUN J, YOU X, LI L, et al. Effects of a phospholipase D inhibitor

on postharvest enzymatic browning and oxidative stress of litchi

fruit[J]. Postharvest Biology and Technology, 2011, 62(3): 288-294.

DOI:10.1016/j.postharvbio.2011.07.001.

[19] CLAUDOT A C, DROUET A. Preparation and assay of chalcone

synthase from walnut tree tissue[J]. Phytochemistry, 1992, 31(10):

3377-3380. DOI:10.1016/0031-9422(92)83689-V.

[20] 张红. 大豆发芽富硒工艺及硒在豆芽中的分布研究[D]. 南京: 南京

农业大学, 2010: 51-52.

[21] 袁兴淼, 张涛, 程须珍, 等. 我国绿豆种质资源的芽用特性评价与

筛选[J]. 植物遗传资源学报, 2012, 13(5): 879-883. DOI:10.3969/

j.issn.1672-1810.2012.05.028.

[22] LEE S, SHEO H, CHOI G, et al. Studies on the performance

characteristics of collected native sprout soybean, Ⅰ. Variation of

characteristics in collected native sprout soybean[J]. Korean Journal of

Breeding, 1992, 24: 87-95.

[23] 薛云皓, 仵红梅, 王爱月, 等. 玉米发芽前后的营养变化[J]. 河南医

学研究, 2001, 3(1): 5-7. DOI:10.3969/j.issn.1004-437X.2001.01.003.

[24] OLOYO R. Chemical and nutritional quality changes in germinating

seeds of Cajanus cajan L.[J]. Food Chemistry, 2004, 85(4): 497-502.

DOI:10.1016/S0308-8146(02)00454-5.

[25] WANASUNDARA P, SHAHIFI F, BROSNAN M. Changes in

flax (Linum usitatissmum) seed nitrogenous compounds during

germination[J]. Food Chemistry, 1999, 65(3): 289-295. DOI:10.1016/

S0308-8146(98)00176-9.

[26] 郑艺梅, 何瑞国, 郑琳, 等. 糙米发芽过程中营养成分及植酸含

量变化的研究[J]. 中国粮油学报, 2006, 21(5): 1-4. DOI:10.3321/

j.issn:1003-0174.2006.05.001.

[27] 石有太, 陈垣, 郭凤霞, 等. 掌叶大黄籽粒营养物质积累动态及其发

芽特性研究[J]. 中国中药杂志, 2009(15): 1979-1983. DOI:10.3321/

j.issn:1001-5302.2009.15.025.

[28] HELLAND M, WICKLUND T, NARVHUS J. Effect of germination

time on alpha-amylase production and viscosity of maize porridge[J].

Food Research International, 2002, 35(2): 315-321. DOI:10.1016/

S0963-9969(01)00202-2.

[29] 郭元新. 盐和低氧胁迫下发芽大豆γ-氨基丁酸富集与调控机理研究[D].

南京: 南京农业大学, 2011: 36-37.

[30] 陈志刚, 顾振新. 温度处理对发芽糙米中淀粉酶活力的影响[J].

食品与发酵工业, 2003, 29(3): 46-49. DOI:10.3321/j.issn:0253-

990X.2003.03.011.

[31] 申海进, 汪海峰, 袁健, 等. 大豆发芽期异黄酮含量变化的研究及其

促进剂的影响[J]. 中国粮油学报, 2007, 21(6): 75-77. DOI:10.3321/

j.issn:1003-0174.2006.06.019.

[32] 徐茂军. 不同品种发芽大豆中异黄酮含量变化的比较

研究[J]. 营养学报, 2002, 24(4) : 444-446. DOI:10.3321/

j.issn:0512-7955.2002.04.029.