Bioactive Peptide Derived from Oyster Meat from Guangxi Region, China Inhibits Proliferation and Migration of Human Ovarian Carcinoma Cells with Directional High Lymphatic Metastasis

doi:10.7506/spkx1002-6630-201613036

Abstract

Abstract:

Objective: To study the inhibitory effect of bioactive peptide derived from oyster meat Guangxi region,
China on the proliferation and migration of human ovarian carcinoma cells with directional high lymphatic metastasis
(SKOV3-PM4 cells). Methods: The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay
was performed to study the inhibitory effect of the bioactive peptide on cell proliferation in 24 h. The proliferative ability
of SKOV3-PM4 cells was detected by cell counting method and colony formation test. The ability of cell migration and
invasion was examined by cell wound scratch assay and matrigel invasion assay. Results: MTT results showed that the
bioactive peptide could inhibit the proliferation of SKOV3-PM4 within the concentration range of 5?80 mg/L and that half
inhibition concentration (IC50) in 24 hours was 35.36 mg/L. The cell growth curve and cell colony formation showed that
the proliferative ability of SKOV3-PM4 cells was significantly inhibited (P < 0.05). Compared with control cells, after
being treated with 4 mg/L (non-cytotoxic concentration) the bioactive peptide, the migration and invasion capability of
SKOV3-PM4 was also significantly decreased (P < 0.05). Conclusion: The bioactive peptide derived from oyster from
Guangxi region can inhibit the proliferation and migration of SKOV3-PM4 cells within a certain concentration range.

Key words: bioactive peptide derived from oyster, ovarian cancer, lymphatic metastasis, cell migration

CLC Number:

R737.31

LI Chaozhu, CHEN Yanhui, CHEN Yanhua, LI Danrong. Bioactive Peptide Derived from Oyster Meat from Guangxi Region, China Inhibits Proliferation and Migration of Human Ovarian Carcinoma Cells with Directional High Lymphatic Metastasis[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201613036.

References

[1] JEMAL A, TIWARI RC, MURRAY T, et al. Cancer statistics,

2004[J]. CA: A Cancer Journal for Clinicians, 2004, 54(1): 8-29.

DOI:10.3322/canjclin.54.1.8.

[2] 乐杰. 妇产科学[M]. 7版. 北京: 人民卫生出版社, 2008: 278．

[3] 周璟, 黄学惠. 卵巢癌的治疗现状回顾[J]. 现代肿瘤医学, 2012,

20(6): 1308-1311. DOI:10.3969/j.issn.1672-4992.2012.06.73.

[4] 蔡福龙, 邵宗泽. 海洋生物活性物质: 潜力与开发[M]. 北京: 化学工

业出版社, 2014: 7.

[5] 陈艳辉, 李超柱, 黎丹戎, 等. 动物蛋白酶解制备广西产牡蛎肉抗肿

瘤活性肽的实验研究[J]. 食品工业科技, 2010, 31(8): 167-169.

[6] 李超柱, 陈艳华, 陈艳辉, 等. 牡蛎活性肽的分离及其免疫抑制作用

的实验研究[J]. 海洋科学, 2013, 37(4): 52-54.

[7] 阮和云, 黎丹戎, 李力, 等. 卵巢上皮性癌淋巴道定向高转移细胞

系的建立及其生物学特性的鉴定[J]. 中华妇产科杂志, 2007, 42(7):

482-486. DOI:10.3760/j.issn:0529-567x.2007.07.013.

[8] 颜克兰, 唐东平, 张传珉, 等. 肿瘤药敏试验与临床病理及疗效

的关系[J]. 中国癌症防治杂志, 2011, 3(1): 23-27. DOI:10.3969/

j.issn.1674-5671.2011.01.07.

[9] 余杰, 杨振国, 陈美珍, 等. 牡蛎活性肽体外诱导HeLa细胞凋亡及其

作用机制[J]. 食品科学, 2012, 33(21): 290-294.

[10] 廖共山, 周先果, 班建东, 等. 牡蛎活性肽对人舌鳞癌Tca8113细胞

增殖和凋亡的影响[J]. 山东医学, 2009, 49(47): 13-15. DOI:10.3969/

j.issn.1002-266X.2009.47.005.

[11] 梁盈, 黄大川, 石松林, 等. 牡蛎低分子活性肽对人肺腺癌A549细胞

形态与超微结构变化的影响[J]. 厦门大学学报(自然科学版), 2006,

45(增刊1): 177-180. DOI:10.3321/j.issn:0438-0479.2006.z1.044.

[12] 李鹏, 李祺福, 石松林, 等. 牡蛎天然活性肽对人胃腺癌BGC-823

细胞周期与基因表达的调控[J]. 中国海洋药物, 2007, 26(3): 1-8.

DOI:10.3969/j.issn.1002-3461.2007.03.001.

[13] FRIEDL P, WOLF K. Tumour-cell invasion and migration: diversity

and escape mechanisms[J]. Nature Reviews Cancer, 2003, 3(5):

362-374. DOI:10.1038/nrc1075.

[14] 高卫栋, 王红兵, 吴泽志, 等. 肿瘤细胞迁移特性及细胞迁移能

力表征[J]. 细胞生物学杂志, 2008, 30(4): 451-456. DOI:10.3969/

j.issn.1674-7666.2008.04.008.

[15] 李南, 韦兴, 陈秉耀, 等. 类间充质干细胞成瘤过程中运动及迁移能

力的变化[J]. 中国骨肿瘤骨病, 2010, 9(4): 342-345. DOI:10.3969/

j.issn.1671-1971.2010.04.017.

[16] 饶毅, 吴瑛. 神经细胞迁移导向的分子机制[J]. 生理科学进展, 2000,

31(3): 198-204.

[17] 唐杰, 陈龙菊, 王军, 等. Transwell和Wound healing在细胞迁移

中的应用比较[J]. 中国临床解剖学杂志, 2008, 25(6): 687-689.

DOI:10.3969/j.issn.1001-165X.2007.06.022.

[18] 刘小慧, 章涛, 张潜, 等. 兔动员外周血间充质干细胞集落形成能

力与神经元诱导分化研究[J]. 西安交通大学学报(医学版), 2014,

35(1): 26-29. DOI:10.7652/jdyxb201401006.

[19] 师忠芳, 韩明, 徐立新, 等. 体外培养大鼠星形胶质细胞划伤模型[J].

中国康复理论与实践, 2007, 13(12): 1132-1133. DOI:10.3969/

j.issn.1006-9771.2007.12.014.

[20] 蒋海鹰, 颜亚晖, 罗庚求, 等. 细胞体外侵袭实验中Transwell plate

染色技术改良[J]. 中国组织化学与细胞化学杂志, 2011, 20(6):

644-645. DOI:10.3870/zgzzhx.2011.06.022.

[21] 纪晓宁, 丰有吉. 上皮性卵巢癌病因学研究进展[J]. 中国癌症杂志,

2014, 24(11): 861-864. DOI:10.3969/j.issn.1674-1870.2010.05.008.

[22] 朱根海, 杨舒盈, 陈春英, 等. 卵巢癌转移灶细胞粘附分子检测及

其腹腔播散机制探讨[J]. 中国优生与遗传杂志, 2000, 8(3): 31-32.

DOI:10.3969/j.issn.1006-9534.2000.03.013.

[23] 田红, 于鹏, 吴小茗, 等. 卵巢癌的治疗药物研究进展[J]. 现代药物与

临床, 2015(1): 103-107. DOI:10.7501/j.issn.1674-5515.2015.01.023.

[24] 柴周芳, 翟东霞, 俞超芹. 卵巢癌靶向药物治疗的研究进展[J]. 中国

妇产科临床杂志, 2014(5): 471-472.

[25] 刘云, 杜成, 刘文超. 卵巢癌治疗新进展[J]. 现代肿瘤医学, 2015,

23(4): 553-556. DOI:10.3969/j.issn.1672-4992.2015.04.36.