Abstract:

Isoquercetin was extracted from Fagopyrum tataricum and investigated for its effect on cell proliferation,
apoptosis and migration of human gastric carcinoma cell line SGC-7901. Cell proliferation was examined by using MTT
assay. The morphological changes of SGC-7901 were observed by DAPI nuclear staining. The effect of Fagopyrum
tataricum isoquercetin on cell migration was investigated by scratch-recovering tests in cell culture plates, as well as its
effect on cell apoptosis by flow cytometry. As a result, MTT assay showed that the isoquercetin could inhibit the viability
of SGC-7901 cells in time- and dose-dependent manners. SGC-7901 cell growth was inhibited by 35.92% or more at
isoquercetin concentration of 100 μmol/L for 48 h, compared to only 3.15% for 293T cells under the same conditions
of concentration and culture duration. DAPI nuclear staining showed chromosomal condensation and the production of
apoptotic bodies. Scratch-recovering tests indicated that the isoquercetin could suppress cell migration. Flow cytometry
showed that the number of cells at the G1 phase and S phase decreased and increased at the G2/M phase and the apoptotic cell
population exhibited a significant increase. Taken together, isoquercetin from F. tataricum can induce apoptosis, arrest cell
cycle and inhibit cell proliferation and migration of SGC-7901 cells.

Key words: isoquercetin, human gastric carcinoma cell line SGC-7901, cell cycle, apoptosis, cell migration