Antitumor Activity and Mechanism of Action of Wild Cordyceps sobolifera Polysaccharide

doi:10.7506/spkx1002-6630-201613038

Abstract

Abstract:

Objective: The antitumor activity of polysaccharides from wild Cordyceps sobolifera (CSP) was investigated in
vitro and in vivo. Methods: 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay was used to
determine the anti-proliferative effect of CSP at different doses on HeLa cells. S180 sarcoma mouse model was established
and used to detect the antitumor activity and the effect on the immune system of CSP. Results: CSP at concentrations of
400?800 μg/mL inhibited the growth of HeLa cells in vitro in a concentration-dependent manner, with a percentage inhibition
of 61% at 800 μg/mL. In vivo, CSP at both high (200 mg/(kg·d)) and low (100 mg/(kg·d)) doses had antitumor effects, with a
percentage inhibition of 42.9% at 200 mg/(kg·d), and immune organ indices of tumor-burdened mice significantly increased
(P < 0.01). CSP also activated the proliferation and differentiation of lymphocytes, and had an obvious protection on the
antioxidant defense system in the body. Conclusion: CSP has obvious antitumor activity in vitro and in vivo, which may be
related to enhancing the immune system adjustment capacity and antioxidant activity.

Key words: Cordyceps sobolifera polysaccharide (CSP), antitumor activity, immune regulation, antioxidant

CLC Number:

R931

XIE Fei, LI Wei, CHEN Meizhen, YU Jie. Antitumor Activity and Mechanism of Action of Wild Cordyceps sobolifera Polysaccharide[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201613038.

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