Abstract:

The amino acid composition of three peptides fractions (TSGH-1, TSGH-2 and TSGH-3) separated from tilapia
skin gelatin hydrolysate (TSGH) was measured. Additionally, the antioxidant activity in vitro and anti-aging capacity
in vivo of TSGH-3 were evaluated. The results showed TSGH-3 had low molecular weight and high hydrophobic amino
acid contents. TSGH-3 had high antioxidant activity in vitro in terms of free radical scavenging and metal chelating
activity. Furthermore, the in vivo anti-aging activity in a D-galactose-induced aging mouse model indicated that TSGH-3
could significantly increase T-AOC, SOD and GSH-Px activities in serum, liver and kidney tissues of rats (P < 0.05), and
significantly decreased MDA contents (P < 0.05). Besides, liver and kidney indices of rats could be also protected after the
intake of TSGH-3. Therefore, TSGH-3 possesses high antioxidant activity and can be used as a natural anti-aging agent in
the medicinal and food industries.

Key words: tilapia skin gelatin hydrolysates, amino acid composition, antioxidant activity, anti-aging