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Analysis of Oligosaccharides Released from Weak Acid Hydrolysis of Abalone Gonad Polysaccharide Using PMP-HPLC-MSn

LU Jiaojiao, AI Chunqing, WEN Chengrong, XU Xin, ZHANG Bao, SONG Shuang   

  1. 1. School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China; 2. National Engineering Research
    Center of Seafood, Dalian 116034, China; 3. National and Local Joint Engineering Laboratory for Marine Bioactive Polysaccharide
    Development and Application, Dalian 116034, China
  • Online:2016-08-25 Published:2016-08-30
  • Contact: SONG Shuang

Abstract:

In order to investigate oligosaccharides released from abalone gonad sulfated polysaccharide (AGSP) through
weak acid hydrolysis, hydrolysates obtained at different acid concentrations were labeled with 1-phenyl-3-methyl-5-
pyrazolone (PMP), and then analyzed by high performance liquid chromatograph-mass spectrometry (HPLC-MSn). Two
disaccharides (DS1 and DS2) and one tetrasaccharide (TS) were found in the AGSP hydrolysates. The two disaccharides
were composed of a hexuronic acid linked to a hexose through a glucosidic bond. DS2 was deduced as β-GlcA(1→2)-Man,
and TS was proposed as a dimer of DS2. In hydrolysis reaction with 0.1–2.0 mol/L trifluoroacetic acid at 100 ℃ for 1 h, the
yields of the three oligosaccharides all increased with increasing acid concentration.

Key words: abalone, polysaccharide, acid hydrolysis, mass spectrometry, oligosaccharide

CLC Number: