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Effect of Transglutaminase on Gel Properties of Wheat Protein and Soybean Protein Mixtures

WANG Yaqiang1, LUO Shuizhong1,2, ZHONG Xiyang1,2, JIANG Shaotong1,2, ZHENG Zhi1,2, ZHAO Yanyan1,2,*   

  1. 1. School of Biology and Food Engineering, Hefei University of Technology, Hefei 230009, China;
    2. Key Laboratory for Agriculture Products Processing of Anhui Province, Hefei 230009, China
  • Online:2016-11-15 Published:2016-11-18

Abstract:

The effect of transglutaminase (TGase) on gel properties of mixed wheat protein and soybean protein were
investigated. In addition, the conformation of mixed protein gels was studied by analyzing surface hydrophobicity, free
sulfhydryl content, thermal properties, secondary structure and microstructure. Results showed that, the gel strength
significantly increased with increased proportion of soybean protein up to 45%. When TGase crosslinked with mixed protein
gels, the thermal stability increased, while the free sulfhydryl content and surface hydrophobicity decreased. Compared with
TGase-treated wheat protein, the gel strength and β-sheet content of the mixed protein gel containing 45% soybean protein
increased by 62.74% and 5.701%, respectively; however, the free sulfhydryl content decreased by 47.48%.

Key words: wheat protein, soybean protein, transglutaminase, gel

CLC Number: