FOOD SCIENCE ›› 2016, Vol. 37 ›› Issue (22): 187-191.doi: 10.7506/spkx1002-6630-201622028

• Safety Detection • Previous Articles     Next Articles

On-Line Detection and Classification of Egg Freshness Based on Consensus Uninformative Variable Elimination-Partial Least Squares-Discriminant Analysis (CUVE-PLS-DA)

WANG Qiaohua1,2, LI Xiaoming1, DUAN Yufei1   

  1. 1. College of Engineering, Huazhong Agricultural University, Wuhan 430070, China;
    2. National Egg Processing Technology Research and Development Sub-centers, Huazhong Agricultural University, Wuhan 430070, China
  • Received:2016-04-17 Online:2016-11-16 Published:2017-02-22

Abstract: Although there are many methods available to detect egg freshness at present, they have shortcomings including laboriousness, low precision and low classification efficiency. An on-line monitoring device based on visible/near infrared spectroscopy (501–1 000 nm) was fitted to the 4 800 eggs per hour egg transport machine for the purpose of dynamically collecting transmittance spectral data for eggs. The collected data were used to establish a partial least squares discriminant (PLS-DA) model for the Haugh unit value of eggs. A total of 226 egg samples were randomly divided into two set: calibration set (n = 169) and validation set (n = 57). By compared different spectral pretreatments and two wavelength selection methods, it was found that standard normal variate (SNV) transformation and multi-pattern consensus method could effectively improve the accuracy, efficiency and predictive ability of the PLS-DA model. The final calibration and validation accuracy were 92.31% and 91.23%, respectively. This study showed that visible-near infared spectroscopy could be used as a real-time and non-destructive detection method to classify egg freshness.

Key words: egg, freshness, online, partial least squares, multi-pattern

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