Abstract: In this study, we used the wild-type Corynebacterium glutamicum strain ATCC 13032 as a starting strain to obtain five mutants with knockout of the ptsG and ptsH-ptsI genes as well as the abc, abc2 and iolt1 genes by homologous recombination without resistance marker using reverse metabolic engineering strategies. Our experimental results showed that compared to the wild-type strain, the glucose consumption of the mutant CGΔptsG was 50% using glucose as the sole carbon source, giving an OD value of 1.473, the glucose consumption of the mutant CGΔptsH-ptsI was 39.5%, giving an OD value of 1.226, the glucose consumption of the mutant CGΔabc was 36%, giving an OD value of 1.09, and the glucose consumption of CGΔabc2 was 26.2%, giving an OD value of 0.486, while the mutant CGΔiolt1 could not utilize glucose to grow, suggesting that glucose metabolism of C. glutamicum was blocked. It turned out that the glucose transporter function was controlled by the ptsG, ptsH-ptsI, abc, abc2 and iolt1 genes, encoding transporter proteins.

Key words: Corynebacterium glutamicum, glucose, gene knockout