Abstract: In this paper, a rapid label-free method to analyze the immune response to allergens by using a portable surface plasmon resonance (SPR) biochip was presented. Shrimp hemocyanin, monoclonal antibody against shrimp hemocyanin, and ascites fluid containing the monoclonal antibody were immobilized onto the surface of the biochip as probes, respectively. The biochip was then used to detect the immune response between shrimp hemocyanin and the corresponding monoclonal antibody. The kinetics of the reaction was analyzed, and the standard curve was constructed. Eight different batches of samples and seven different batches of unpurified ascites were detected on the same chip. This method allowed real-time, label-free, low-cost, environment-friendly and easy-to-control detections. It was suitable for real-time and continuous detection and rapid screening of a large number of samples. The developed portable SPR biochip detection system can be widely applicable to real-time rapid detection and quality control in factories, markets, supermarkets and other fields. It can also be applied in the clinical detection of allergen-specific antibody in serum of patients.

Key words: allergen, surface plasmon resonance, biochip, label-free, rapid detection, immunoreaction