Abstract: This experiment explored the effect of kidney bean sprout polyphenols on restoring the antioxidant system and D-galactose-induced liver injury in mice. Intraperitoneal injection of D-galactose solution was conducted to construct a mouse model of oxidative injury. Body weight, liver coefficient and superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), alanine aminotransferase (ALT) and aspartate transaminase (AST) activities and malondialdehyde (MDA) concentration in serum were determined after 28 days of continuous administration of different doses (20、40、60 mg/(kg·d)) of kidney bean sprout polyphenols, and liver tissue sections were stained with hematoxylin-eosin and examined for the degree of liver damage and recovery. Compared with the model control group, oral administration of kidney bean sprout polyphenols showed no significant differences in body weight or liver coefficient (P > 0.05); however, serum SOD and GSH-Px activities in the middle- and high-dose groups were increased significantly (P < 0.05), MDA concentration was decreased significantly (P < 0.05), and the liver function indicators ALT and AST activities were reduced significantly (P < 0.05). Histopathological observation indicated that swollen hepatocytes were restored to normal by the administration of kidney bean sprout polyphenols and the number of intercellular bleeding points and inflammatory cell infiltration were reduced dose-dependently.

Key words: kidney bean sprout polyphenols, D-galactose, oxidative damage, liver, restoring effect