FOOD SCIENCE ›› 2017, Vol. 38 ›› Issue (24): 265-271.doi: 10.7506/spkx1002-6630-201724043

• Safety Detection • Previous Articles     Next Articles

Simultaneous Determination of 55 Plastic Additives Migrating from Plastic Containers to Yogurt by Solid Phase Extraction-Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

BO Haibo, XING Yuxiu, JI Shengjun, WANG Jingying, YANG Juan   

  1. (1. College of Chemistry and Chemical Engineering, Qinghai Normal University, Xining 810008, China;2. Security Department of Criminal Science and Technology, Qinghai Public Security Bureau, Xining 810000, China)
  • Online:2017-12-25 Published:2017-12-07

Abstract: A method has been developed to determine 55 plastic additives including antioxidants, plasticizers, ultraviolet light absorbers in yogurt by solid phase extraction-ultra performance liquid chromatography-tandem mass spectrometry (SPE-UPLC-MS/MS). Samples were extracted with acetonitrile and the extract was then cleaned up on an HLB SPE column. The UPLC system equipped with a ACQUITY UPLC BEH C18 analytical column (2.1 mm × 50 mm, 1.7 μm) was operated utilizing methanol-0.1% formic acid as the mobile phase in gradient elution mode. Electrospray ionization in positive ion mode (ESI+) with multiple reaction monitoring (MRM) was used, and the analytes were quantified by the external standard method. The calibration curves displayed good linearity in a wide concentration range with a correlation coefficient of over 0.97. The average recoveries from spiked yogurt at three concentrations ranged from 62.4% to 93.5% with relative standard deviations (RSD) varying from 2.4% to 13.4%. The limits of detection (LOD) were 0.2–40 μg/kg. Twenty-three plastic additives at concentrations from 7.0 to 39 μg/kg were detected in 20 samples by the proposed method. This method can simultaneously identify and quantify a wide variety of plastic additive compounds with distinctive physicochemical properties in yogurt with high sensitivity.

Key words: plastic additives, ultra performance liquid chromatography-tandem mass spectrometry, high throughput detection, yogurt

CLC Number: