Abstract: The in vivo and in vitro antioxidant effects of soluble dietary fiber (SDF) from mung bean (Phaseolus radiates L.) hull were studied. The results showed that the reducing power of SDF at a concentration of 4 mg/mL was 1.526, which could scavenge 82.65% 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and 85.16% hydroxyl radical. Animal experiments showed that total antioxidant capacity (T-AOC), catalase, total superoxide dismutase, glutathione peroxidase activity in the serum and liver tissue of D-Galactose-induced aging mice decreased, while malondialdehyde (MDA) content increased with significant differences being observed for most parameters (P < 0.05 or P < 0.01) when compared with the normal control group, indicating that the animal model of D-galactose-induced aging was constructed successfully. Compared with the model group, MDA content in the serum and liver tissue of mice administrated with SDF from all dose groups significantly decreased. High-dose SDF could significantly improve T-AOC and total superoxide dismutase, catalase, glutathione peroxidase activity in the serum and liver tissue of mice (P < 0.05). Therefore, SDF from mung bean hull has an excellent antioxidant activity.

Key words: mung bean hull, soluble dietary fiber, D-galactose, antioxidant activity