Visual Detection of Pork Components in Common Meat Products by Loop-Mediated Isothermal Amplification (LAMP) Method

doi:10.7506/spkx1002-6630-20180526-369

Abstract

Abstract: Six primers specific to the mitochondrial cytochrome b gene of swine were designed for use in loop-mediated isothermal amplification (LAMP). A simplified procedure was used for mitochondrial DNA extraction, and the reaction system was optimized. The specificity was tested with mitochondrial DNA samples from beef, mutton, chicken, duck meat, horse meat, and donkey meat as the negative controls, and the limit of detection (LOD) was determined with gradient dilution of adulterated meat with pork. A visual LAMP method for the detection of pork components in common meat products was established. The results showed that the modified mitochondrial DNA extraction method was simple and repeatable, and the visual LAMP method with 4-(2-pyridylazo)-resorcinol sodium salt as the indicator could accurately detect pork adulteration in common meat products with an LOD of 10 pg/μL.

Key words: loop-mediated isothermal amplification, pork, detection, mitochondrial DNA extraction, visualization

CLC Number:

TS207.3

ZHU Kai, KANG Huaibin, WANG Deguo. Visual Detection of Pork Components in Common Meat Products by Loop-Mediated Isothermal Amplification (LAMP) Method[J]. FOOD SCIENCE, 2019, 40(12): 296-302.

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Visual Detection of Pork Components in Common Meat Products by Loop-Mediated Isothermal Amplification (LAMP) Method

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