Abstract: Cordycepin and adenosine were simultaneously extracted from the solid-state culture of Cordyceps militaris with rice and brewer’s spent grain (BSG) as co-substrates. The extraction process was optimized using one-factor-at-a time method and response surface methodology (RSM). The total yield of cordycepin and adenosine was investigated as a function of four variables including extraction time, ethanol concentration, solvent-to-solid ratio and temperature. The optimal extraction conditions were found to be extraction at 45 ℃ for 87 min with water as a solvent at a solvent-to-solid ratio of 22:1 (mL/g). Under these conditions, The maximum total yield (2.491‰) of cordycepin and adenosine was experimentally obtained, which agreed with the predicted value (2.487‰). Accordingly, the regression model developed showed excellent goodness of fit. The solid-state culture of Cordyceps militaris is a promising rich of cordycepin and adenosine.

Key words: Cordyceps militaris, solid-state culture, simultaneous extraction, response surface methodology