Separation and Identification of Metabolites and Metabolic Mechanism in Two Cultivars of Mung Bean

doi:10.7506/spkx1002-6630-20200303-044

FOOD SCIENCE ›› 2021, Vol. 42 ›› Issue (16): 169-175.doi: 10.7506/spkx1002-6630-20200303-044

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Separation and Identification of Metabolites and Metabolic Mechanism in Two Cultivars of Mung Bean

ZHANG Liyuan, YU Yingbo, ZHAO Ziying, YU Runzhong, LI Zhijiang, ZHANG Dongjie

(1. College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China;2. College of Electrical and Information, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 3. Cultivating Collaborative Innovation Center for the Beidahuang, Daqing 163319, China; 4. Heilongjiang Engineering Research Center for Coarse Cereals Processing and Quality Safety, Daqing 163319, China; 5. Chinese National Engineering Research Center, Daqing 163319, China)

Published:2021-08-27

Abstract

Abstract: Based on metabonomics, the metabolites in two mung bean cultivars (Daming and Jiulihu) were isolated and identified by gas chromatography-mass spectrometry (GC-MS). The polar metabolits were extracted with aqueous methanol solution and then derivatized with N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) before being analyzed by GC-MS. The results showed that a total 67 metabolites were identified in the mung bean cultivars, 30 of which were found to be common to be both, but at different levels. Ten differential metabolites were identified in Daming mung beans compared with Jiulihu mung beans, and 20 differential metabolites in Jiulihu mung beans compared with Daming mung beans. The metabolic pathways of sugar, fatty acid, amino acid and TCA in Jiulihu mung beans were more active than in Daming mung beans. The dominant metabolites (> 7%) in Daming mung beans were L-norvaline and inositol. The dominant metabolites in Jiulihu mung beans was L-norvaline. We speculated that the difference in the metabolite composition between the two cultivars may be related to the difference in genes, metabolic pathways and other factors.

Key words: green bean; metabolite; gas chromatography-mass spectrometry; metabonomics; different cultivars

CLC Number:

O657.63

ZHANG Liyuan, YU Yingbo, ZHAO Ziying, YU Runzhong, LI Zhijiang, ZHANG Dongjie. Separation and Identification of Metabolites and Metabolic Mechanism in Two Cultivars of Mung Bean[J]. FOOD SCIENCE, 2021, 42(16): 169-175.

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Separation and Identification of Metabolites and Metabolic Mechanism in Two Cultivars of Mung Bean

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