Antibacterial Activity and Mechanism of Terpinene-4-ol against Pseudomonas fluorescens

doi:10.7506/spkx1002-6630-20201113-143

FOOD SCIENCE ›› 2022, Vol. 43 ›› Issue (1): 30-36.doi: 10.7506/spkx1002-6630-20201113-143

Antibacterial Activity and Mechanism of Terpinene-4-ol against Pseudomonas fluorescens

GENG Yiming, LI Tingting, LI Jianrong, XIE Jing, LIN Hong, WANG Hong, SHEN Zhaohua, GUO Xiaohua, LAO Minjun, ZHOU Xiaomin, YU Jianyang

(1. National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, College of Food Science and Engineering, Bohai University, Jinzhou 121013, China; 2. College of Life Science, Dalian Minzu University, Dalian 116600, China; 3. College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, China; 4. College of Food Science and Engineering, Ocean University of China, Qingdao 266100, China; 5. Penglai Jinglu Fishery Co., Ltd., Yantai 265600, China; 6. Shandong Meijia Group Co., Ltd., Rizhao 276800, China; 7. Zhejiang Xingye Industrial Group Co., Ltd., Zhoushan 316120, China; 8. Rongcheng Taixiang Food Co., Ltd., Key Laboratory of Frozen Prepared Marine Food Processing, Ministry of Agriculture and Rural Affairs, Weihai 264309, China）

Published:2022-01-29

Abstract

Abstract: The antibacterial activity of terpinene-4-ol against Pseudomonas fluorescens was investigated by measuring the minimum inhibitory concentration (MIC) and growth curve. Its effect on cell wall and cell membrane permeability were evaluated by determination of alkaline phosphatase activity and electrical conductivity, fluorescein diacetate (FDA) staining and scanning electron microscope (SEM) observation, and its effect on intracellular macromolecules and cellular metabolism was further explored by determining DNA, total protein and Na+, K+-ATPase activity. The results showed that the MIC was determined to be 2 μL/mL. Terpinen-4-ol could effectively destroy the cell wall of P. fluorescens in a dose-dependent manner, and enhance cell membrane permeability, leading to the leakage of intracellular ions. The results of SEM showed that terpinen-4-ol could damage the cell membrane irreversibly. Gel retardation electrophoresis analysis revealed that terpinene-4-ol could cause leakage of macromolecular DNA. Additionally, terpinen-4-ol reduced intracellular protein content and blocked the expression of protein and the synthesis of ATPase, thus resulting in cell apoptosis. Therefore, terpinen-4-ol has the potential to be used as a new preservative for aquatic products.

Key words: terpinene-4-ol; Pseudomonas fluorescens; antibacterial activity; antibacterial mechanism

CLC Number:

TS201.3

GENG Yiming, LI Tingting, LI Jianrong, XIE Jing, LIN Hong, WANG Hong, SHEN Zhaohua, GUO Xiaohua, LAO Minjun, ZHOU Xiaomin, YU Jianyang. Antibacterial Activity and Mechanism of Terpinene-4-ol against Pseudomonas fluorescens[J]. FOOD SCIENCE, 2022, 43(1): 30-36.

[1]	Bo YANG. Screening of starter culture of excellent lactic acid bacteria from donkey milk source in Xinjiang area and its probiotic characteristics [J]. , 0, (): 0-0.
[2]	Shang-Chao MA Kai-ju Mo. Analysis of Bacterial Diversity in Enshi Prefecture Sojae Semen Praeparatum Based on High-throughput Sequencing Technology [J]. FOOD SCIENCE, 0, (): 0-0.
[3]	. Antibacterial activity and mechanism of Terpinene-4-ol against Pseudomonas fluorescens [J]. , 0, (): 0-0.
[4]	Ruiling Lv Ding Tian Jianwei Zhou Donghong LIU. Effect and mechanism of ultrasound in the inactivation of bacterial spores [J]. , 0, (): 0-0.
[5]	慎敏康 Rui-Yun WU. The Application of Lactic Acid Bacteria Against Foodborne Pathogens: A Review [J]. FOOD SCIENCE, 0, (): 0-0.
[6]	Yin-Hui LI Wenjun Wang Ruiling Lv Donghong LIU. Progress in research on the combination treatment of ultrasound with bactericide [J]. FOOD SCIENCE, 0, (): 0-0.
[7]	Li-Jun TAN Qiao-Hui ZENG Yong Zhao. Mixed-species biofilms formation, interaction and novel control strategies in the food industry [J]. FOOD SCIENCE, 0, (): 0-0.
[8]	Deng Gaowen JIANG Li-wen. Analysis of Bacterial Diversity in Guizhou Water-Douchi Based on Illumina MiSeq High-throughput Sequencing Technology [J]. , 0, (): 0-0.
[9]	Ming-Xun CUI. Analysis of bacterial community structure in the processing of low sodium Western fermented ham [J]. , 0, (): 0-0.
[10]	. Effect of Temperatures on the Color, Physicochemical Characteristics and Antioxidant Activities of Ovalbumin-Glucose System under the Action of Alkali [J]. FOOD SCIENCE, 0, (): 0-0.
[11]	JIA Rui, CAI Dan, GE Sitong, REN Shida, LIU Jingsheng. Antibacterial Activity and Mechanism of Polyphenol Extracts from Adzuki Bean Seed Coat against Two Pathogens [J]. FOOD SCIENCE, 2021, 42(23): 64-71.
[12]	. Structural characteristics, recombination strategies of phage lysins and its application in controlling foodborne pathogens [J]. , 0, (): 0-0.
[13]	LI Yufang, YANG Kun, GU Weiwei, ZHAO Qiong, HUANG Aixiang, SHI Yanan. Multi-target Antibacterial Mechanism of Milk-derived Antimicrobial Peptide BCp12 against Staphylococcus aureus [J]. FOOD SCIENCE, 2021, 42(23): 34-41.
[14]	GUO Mingmei, KONG Yanhui, LI Xiaohe, DU Zhilei, FAN Xinguang, GONG Hansheng. Analysis of Sites of Action of Phenyllactic Acid in Inhibiting Alternaria sp. [J]. FOOD SCIENCE, 2021, 42(23): 51-56.
[15]	ZHAO Wei, WANG Yangmei, PAN Yingjie,, ZHAO Yong, LIU Haiquan. Progress in the Application of Simulated Gastrointestinal Models to Study the Heterogeneity of Foodborne Pathogens [J]. FOOD SCIENCE, 2021, 42(23): 268-274.

Antibacterial Activity and Mechanism of Terpinene-4-ol against Pseudomonas fluorescens

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments