Discrimination of Not from Concentrate and from Concentrate Orange Juice by Polymerase Chain Reaction and Capillary Electrophoresis

doi:10.7506/spkx1002-6630-20210615-166

FOOD SCIENCE ›› 2022, Vol. 43 ›› Issue (10): 289-294.doi: 10.7506/spkx1002-6630-20210615-166

• Safety Detection • Previous Articles Next Articles

Discrimination of Not from Concentrate and from Concentrate Orange Juice by Polymerase Chain Reaction and Capillary Electrophoresis

SUN Ruixue, XING Ranran, ZHANG Jiukai, GE Yiqiang, ZHANG Weiwei, CHEN Ying

(1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; 2. Chinese Academy of Inspection and Quarantine, Beijing 100176, China; 3. China Rural Technology Development Center, Beijing 100045, China; 4. College of Science, China Agricultural University, Beijing 100083, China)

Online:2022-05-25 Published:2022-05-27

Abstract

Abstract: In the present study, three orange cultivars (Citrus sinensis L. Osbeck), including Trovita, Early-golden, and Hamlin, were selected to produce not from concentrate (NFC) orange juice and from concentrate (FC) orange juice by the commercial manufacturing processes. Eight pairs of primers to obtain targeted fragments of different lengths were designed based on the chloroplast maturase K (matK) and nicotinamide adenine dinucleotide dehydrogenase (ndhF) genes. Polymerase chain reaction (PCR) coupled with capillary electrophoresis (CE) was used to analyze the effects of key processing steps such as extraction, homogenization, pasteurization, concentration, and secondary pasteurization on DNA degradation in the chloroplast matK and ndhF genes in Trovita orange juice. By comparing the differences in DNA degradation between NFC and FC orange juice, we selected the characteristic differential fragment sets and established a PCR-CE method to discriminate between NFC and FC orange juice. The developed method was applicable to analyze NFC and FC orange juice within shelf life. It was equally applicable to discriminate between NFC and FC orange juice manufactured from Early Golden and Hamlin sweet oranges. Finally, this method was used to test commercial NFC orange juice. The results were not consistent with the label information. The PCR-CE method will provide technical support for the effective regulation of the juice industry.

Key words: not from concentrate; from concentrate; polymerase chain reaction-capillary electrophoresis; adulteration; authentication

CLC Number:

TS255.44

SUN Ruixue, XING Ranran, ZHANG Jiukai, GE Yiqiang, ZHANG Weiwei, CHEN Ying. Discrimination of Not from Concentrate and from Concentrate Orange Juice by Polymerase Chain Reaction and Capillary Electrophoresis[J]. FOOD SCIENCE, 2022, 43(10): 289-294.

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Discrimination of Not from Concentrate and from Concentrate Orange Juice by Polymerase Chain Reaction and Capillary Electrophoresis

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