FOOD SCIENCE ›› 2022, Vol. 43 ›› Issue (20): 44-52.doi: 10.7506/spkx1002-6630-20210917-203

• Food Chemistry • Previous Articles     Next Articles

Hydrazide-Assisted Directional Antibody Conjugation of Gold Nanoparticles to Enhance the Performance of Immunochromatographic Assay

ZHOU Mengjun, ZHOU Shu, HUANG Xiaolin, LI Xiangmin, XIONG Yonghua   

  1. (1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China;2. College of Food Science and Technology, Nanchang University, Nanchang 330031, China)
  • Online:2022-10-25 Published:2022-10-26

Abstract: Anti-zearalenone monoclonal antibodies (mAbs) were directionally conjugated on the surface of hydrazide group-functionalized gold nanoparticles because of the nucleophilic addition reaction between the aldehyde groups of the antibody Fc region and the hydrazide groups on gold nanoparticles (AuNPs). Compared with the carbodiimide-mediated covalent coupling method, the proposed directional coupling strategy showed several advantages such as use of less amounts of labeled antibody and shorter reaction time. The hydrazided gold nanoparticles-based immunochromatographic assay (hAuNPs-ICA) showed higher sensitivity for ZEN detection. Additionally, when hAuNPs-ICA was used to analyze ZEN-spiked maize samples, the average recoveries ranged from 83.1% to 118.0% with intra- and inter-batch coefficients of variation ranging from 3.9% to 13.1%, indicating good accuracy and precision.

Key words: immunoprobe; hydrazide; directional coupling; colloidal gold nanoparticles; zearalenone

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