Abstract: With the rapid development of social economy and the continuous improvement of people’s living standards, the demand for foods of animal origin is also increasing. The problem of veterinary drug residues in foods of animal origin has attracted more and more attention. Meanwhile, higher requirements have been raised for the supervision and inspection of veterinary drug residues. High-resolution mass spectrometry (HRMS) is a powerful tool to retrieve compounds, which can enable the non-targeted screening and confirmation of a variety of veterinary drugs without the use of standards. HRMS is an important technical means for risk monitoring and high-throughput screening of veterinary drug residues, making up for the weaknesses of triple quadrupole mass spectrometry such as poor universality and no ability to screen unknown compounds. This review summarizes the application of liquid chromatography-high resolution mass spectrometry (LC-HRMS) in the field of veterinary drug residue detection from the year 2018 to 2021, and systematically demonstrates the advantages and characteristics of HRMS. With the popularization of HRMS and the development of detection technologies, HRMS will be widely used in the field of veterinary drug residue detection as a routine method.

Key words: liquid chromatography; high-resolution mass spectrometry; time-of-flight mass spectrometry; orbitrap mass spectrometry; veterinary drug residues