FOOD SCIENCE ›› 2023, Vol. 44 ›› Issue (24): 34-40.doi: 10.7506/spkx1002-6630-20230404-032

• Food Chemistry • Previous Articles     Next Articles

Evaluation of Stability and Biocompatibility of Chitosan/Sodium Tripolyphosphate and Chitosan/Flaxseed Gum Composite Nanoparticles Loaded with Bighead Carp Peptides

ZHENG Changliang, CHEN Mengting, WANG Lan, QU Yinghong, SHI Wenzheng, SHI Liu, CHEN Sheng, QIAO Yu, LI Xin, GUO Xiaojia, WU Wenjin, YANG Yuping   

  1. (1. Institute of Agricultural Products Processing and Nuclear Agricultural Technology, Hubei Academy of Agricultural Sciences, Key Laboratory of Cold Chain Logistics Technology for Agro-product, Ministry of Agriculture and Rural Affairs, Wuhan 430064, China;2. College of Food Sciences and Technology, Shanghai Ocean University, Shanghai 201306, China;3. Wuhan Institute for Drug and Medical Device Control, Wuhan 430075, China)
  • Online:2023-12-25 Published:2024-01-02

Abstract: Chitosan nanoparticle is becoming an excellent carrier for the delivery of bioactive components due to the advantages of simple preparation, low cost and high biocompatibility. Previous studies have shown that chitosan/sodium tripolyphosphate (CS/TPP) and chitosan/flaxseed gum (CS/FG) nanoparticles loaded with bighead carp peptides (BCP) have the advantages of small particle size, high encapsulation rate and significant slow-release effect. This study explored the effects of ionic strength, pH, simulated digestion and storage time on the preparation of chitosan/sodium tripolyphosphate (CS/TPP-BCP) and chitosan/flaxseed gum (CS/FG-BCP) nanoparticles, and evaluated the extracellular lactate dehydrogenase content and antioxidant capacity in vivo of Caco-2 cells treated with the chitosan nanoparticles and their cellular uptake. The results showed that the two kinds of chitosan nanoparticles were stable under acidic conditions and sensitive to a solution with opposite charges. The stability of the nanoparticles loaded with bighead peptides was higher than that of free peptides and both nanoparticles showed higher biocompatibility and cell uptake.

Key words: chitosan; nanoparticle; stability; biocompatibility

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