Abstract: In this paper the immobilization of alcalase and the properties of immobolized alcalase were studied and the preparation of CPP casein phospospeptides using immobilized alcalase was also investigated. Polystyrene anion ex change resin CM201 and Chitosan used separaely as immobilized enzyme supports and glutaraldehyde as crosslinking agent were introduced to prepare the immobilized 2709 alcalase. The effect of the glutaraldehyde concentration the ratio of alcalase to support on immobilized enzymes and their optimal temperature and pH values were investigated respectively. The Experiment results have showed that the maximum activity retention value was over 19%with the polystyrene anion ex change resin GM201 as the support and over 66%with the chitosan as support. The study also showed that the optimum temperature pH and substrate concentration were 45℃.8.0 and 4.0% respectively. The yield of the CPP useing immo- bilized alcalase to hydrolyze casein reached over 7.54%.

Key words: Support, Casein, Chitosan, Immobilized, Alcalase, Casein Phosphopeptide(CPP)