Abstract: Pectinase was immobilized on chitosan vector by cross likage of glutaraldehyde. Effects of concentration of glutaraldehyde, amount of the enzyme and temperature on immobilization were analyzed. The enzymatic conditions (optimum pH and temprerature ), Km value and half-life of immobilized pectinase were measured. The results showed that the high rate of recovery of immobilized enzyme could be obtained under the conditions of 3% glutaraldehyde, 0.1 mg pectinase/wet chitosan and 5 ℃ temperature. The extent of the optimum temperature of immobilized pectinase was larger than non-immobilized pectinase. The optimum pH and apparent Km value of the immobilized enzyme decreased slightly. Under the 4 ℃ condition, the half-life of immobilized pectinase was 30 d.

Key words: Pectinase , Immobilization , Chitosan