Enzyme Breeding and Producing Conditions of α-Acetylactate Decarboxylase Strain

Abstract

Abstract: A high yield α-acetylactate decarboxylase producing strain was obtained by selecting and compound mutagenesis of UV and NTG with 9 soil Bacillus Lichenforms as starting strain.Among 9 native strains,BL-4 had the highest production of α-acetylactate decarboxylase.After UV mutagenesis of BL-4,the production of α-acetylactate decarboxylase of selected mutated strain(BL-4-UV-3) increased 43.3%.Then BL-4-UV-3 took place the second mutagenesis under NTG,the production of α-acetylactate decarboxylase of selected mutated strain (BL-4-UV-3-NTG-11) increased 1.22 times compared to native strain BL-4.The optimum enzyme producing conditions were 30℃,initial pH at 6.5,and 36h fermentation.

Key words: Bacillus Lichenforms, α-acetylactate decarboxylase , Mutagenesis , Ferment

ZHOU Wen-Bin. Enzyme Breeding and Producing Conditions of α-Acetylactate Decarboxylase Strain[J]. FOOD SCIENCE, 2002, 23(10): 57-59.

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Enzyme Breeding and Producing Conditions of α-Acetylactate Decarboxylase Strain

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