Abstract: Thisbiosensorconsistedoftwoimmo bilizedenzymeelectrodes,oneofwhichwasglucoseoxidaseforglucose detection and the other was an integrated membrane of invertase,mutarotase and glucose oxidase for sucrose d etection.Glucose and sucrose could be determined simultaneously with injec tion amount 25μl and response time 30sec-onds.The biosensor showedgoodlinearity among the range of0～400mg /dl ofsucrose and0～200mg /dl ofglucose.The relative response deviationofsucrose andglucose was 1.13%and1.05%respectively in20assays.The reco very rate of sucrose was 99.62～100.97%.The membrane could be usedcontinuously for two weeks.

Key words: Enzyme electrode , Glucose , Sucrose , Food industry