FOOD SCIENCE ›› 2002, Vol. 23 ›› Issue (9): 91-93.

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Determine Amino Acid Contents with OPA-FMOC Pre-column Derivatization RP-HPLC

 YE  Ying   

  • Online:2002-09-15 Published:2011-12-31

Abstract: A method using pre-column derivatization with o-phthaldialdehyde(OPA) and fluorenylmethyl chloroformate(FOMC chloride)was reported for the determination of amino acids in sample by RP-HPLC.The analysis could be accomplished within 17.0 min by using Hypersil C18. RP-HPLC followed by FLD detection. The method possesses the advantages of effective separation, good reproducibility and satisfactory linear range.

Key words: RP-HPLC Pre-column , Derivatization , Amino acid