Study on Cloning and Expression of the β-galactase Gene from Streptococcus Lactis 6030 in Esherichia Coli

Abstract

Abstract: Lactose metabolism in Streptococcus lactis 6030 was associated with a large plasmid bank. The genes encoding β-galactase from the plasmid of S. lactis 6030 was cloned into the vector pUCl9 in Esherichia coli. The restriction endonuclease map of the 5.0kb PstI insert fragment was analyzed. The recombinant plasmid was expressed in E.coli by IPTG induction,and theβ-galactase production level and activity were determined by the ONPG method.

Key words: Streptococcus Lactis, β-galactase gene, cloning and expression

RAN Xue-Qin, WANG Jia-Fu, WU Yong-Jun, ZHU Qiu-Jin. Study on Cloning and Expression of the β-galactase Gene from Streptococcus Lactis 6030 in Esherichia Coli[J]. FOOD SCIENCE, 2003, 24(2): 26-28.

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Study on Cloning and Expression of the β-galactase Gene from Streptococcus Lactis 6030 in Esherichia Coli

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