Study on Cloning and Expression of A Full-length Gene of Allergenic Protein of Tartary Buckwheat and Its Immunological Activity

doi:10.7506/spkx1002-6630-200901048

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (1): 203-206.doi: 10.7506/spkx1002-6630-200901048

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Study on Cloning and Expression of A Full-length Gene of Allergenic Protein of Tartary Buckwheat and Its Immunological Activity

ZHANG Xin1，CUI Xiao-dong1，LI Yu-ying1，ZHANG De-mei2，WANG Zhuan-hua1,*

(1.Key Laboratory of Chemical Biology and Molecular Engineering, Ministry of Education, Institute of Biotechnology,
Shanxi University, Taiyuan 030006, China ；2.Taiyuan Red-Cross Blood Center, Taiyuan 030001, China)

Received:2008-01-07 Revised:2008-08-04 Online:2009-01-01 Published:2010-12-29
Contact: WANG Zhuan-hua1 E-mail:zhwang@sxu.edu.cn

Abstract

Abstract:

Buckwheat is a common allergen in plants. In the present study, a full-length cDNA encoding 515 amino acid residues was amplified and cloned from the total RNA of tartary buckwheat by PCR and RACE methods. Its nucleotide sequence has high homology with main allergenic storage protein mRNA in common buckwheat. The recombinant allergenic protein of tartary buckwheat (rTBt) expressed in Escherichia.coli BL21 (DE3) was purified by using affinity chromatography and it can specifically bind to the IgE in patients serum with protein allergy of tartary buckwheat. These results make an important foundation for further study about the mechanism and function of the major allergenic protein in tartary buckwheat.

Key words: tartary buckwheat, allergenic protein, cloning and expression, immunological activity

CLC Number:

Q78
R392

ZHANG Xin1，CUI Xiao-dong1，LI Yu-ying1，ZHANG De-mei2，WANG Zhuan-hua1,*. Study on Cloning and Expression of A Full-length Gene of Allergenic Protein of Tartary Buckwheat and Its Immunological Activity[J]. FOOD SCIENCE, 2009, 30(1): 203-206.

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Study on Cloning and Expression of A Full-length Gene of Allergenic Protein of Tartary Buckwheat and Its Immunological Activity

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