Progress in Cloning and Expression of Protease Genes

doi:10.7506/spkx1002-6630-201523067

Abstract

Abstract:

Although proteases are widely applied in various fields, large-scale industrial application of proteases has been
restricted by many factors. Genetic engineering technology has been used for the cloning and expression of protease genes to
obtain the protease resources with high quality, high purity and high activity. The classification, structures and functions of
proteases, and strain screening for protease production are reviewed in this article. Meanwhile, the cloning, and prokaryotic
and eukaryotic expression of protease genes are discussed. Moreover, the future development in protease gene engineering
technology is also prospected.

Key words: protease, genetic engineering technology, cloning and expression

CLC Number:

Q786

WANG Jilian, LI Mingyuan, CHEN Yun. Progress in Cloning and Expression of Protease Genes[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201523067.

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Progress in Cloning and Expression of Protease Genes

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