Study on Screening of a Bacillus Subtilis Strain to Produce Debittering Soybean Peptides and Optimizing Its Liquid Fermention Conditions

Abstract

Abstract: One strain, being fit for the hydrolysis of defatted soybean, was screened out from eight Bacillus subtilis strains. Through the orthogonal test, the optimum conditions of hydrolysis were ascertained: 6%defatted soybean, natural pH tempreture 30℃,and the time of fermentation 48h. After analyzing the free amino acids of B.subtilis broth hydrolyzed by one kind of carboxypeptidase from an Aspergillus strain, some specific sites of the proteases were found.

Key words: Bacillus subtilis, soybean peptide, carboxypeptidase

WAN Qi, LU Zhao-Xin, GAO Hong. Study on Screening of a Bacillus Subtilis Strain to Produce Debittering Soybean Peptides and Optimizing Its Liquid Fermention Conditions[J]. FOOD SCIENCE, 2003, 24(2): 29-32.

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Study on Screening of a Bacillus Subtilis Strain to Produce Debittering Soybean Peptides and Optimizing Its Liquid Fermention Conditions

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