Enzyme Activity Study on Alkalase 2709 in Reverse Micelles

Abstract

Abstract: The enzyme activity of alkalase 2709 incorporated in AOT/iso-octane reverse micelles has been investigated. Effects of substrate concentration, rev erse micelles concentration, W0, pH, temperature, salt concentration and of vari ous kinds were studied in detail. The enzyme showed "super activity" and acted i n the membrane of reverse micelles. When the concentration of AOT/iso-octane wa s 0.08g/ml, factors affecting the activity of the enzyme were as follows: sal t concentration > W0 >pH value >temperature with optimal activity at 9.27×105U ,4 5℃,0.2mol/L KCl, pH 9.2 andW0 value 19.3.

Key words: reverse micelles, alkalase 2709, enzyme activity

YANG Hong-Shun, CHEN Fu-Sheng, LI Yun-Fei, LIU Fang. Enzyme Activity Study on Alkalase 2709 in Reverse Micelles[J]. FOOD SCIENCE, 2003, 24(4): 19-22.

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Enzyme Activity Study on Alkalase 2709 in Reverse Micelles

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