Abstract: The propertie of glucose oxidase covalently immobilized on glutarald e h yde-treated chitosan membrane change.The optimum pH value for Immobilized glu co se oxidase (IGOD) and glucose oxidase(GOD)are 5.5;The optimum temperature fo r I GOD and GOD were 40℃ and 35℃,respectively. The apparent Km of IGOD is 12. 06 mmol/L while Km of GOD is 20.86mmol/L:the thermal stability of IGOD is im prov ed by immobilizati on. The immobilized enzyme had good storage and operatio n st ability and reusability and that properties offer potential for further pra ctica l application.

Key words: Glucose Oxidase, immobilized enzyme, chitosan membrane, properties