FOOD SCIENCE ›› 2003, Vol. 24 ›› Issue (9): 76-79.

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Optimum Conditions Study on Transfer of Linoleic Acid to Conjugated One by Lactobacillus acidophilus

 CAO  Jian, WEI  Ming, ZENG  Shi, CHEN  Xiu-Jin   

  • Online:2003-09-15 Published:2011-12-13

Abstract: A Lactobacillus acidophilus mutant was obtained by ultraviolet radiation, which could transfer linoleic acid toconjugated linoleic acid. The optimum conditions were as follows: reaction temperature 35℃, pH 4.0, incubation time 48h, andthe proportion of linoleic acid and culture medium 1000mg:100ml. About 38.1% linoleic acid was transferred to conjugatedlinoleic acid under such conditions. The yield of conjugated linoleic acid could be improved by Na+, Fe2+ and Mg2+, while inhibitedby Hg2+, Co2+, Cu2+, Mn2+ and Fe3+, taking the concentration of 1mmol/L. The yield of conjugated linoleic acid was also enhancedapparently by ultrasonic treatment.

Key words: Lactobacillus acidophilus, linoleic acid, conjugated linoleic acid, transfer