Separation of Daidzin and Genlstin from Soybean Isoflavones by High Speed Countercurrent Chromatography

Abstract

Abstract: Semlpreparation high speed coufltercurent chromatography(HSCCC)was used for the systematic optimization andpreparation of the two-phase solvent system to separate Daidzin and Genistin from soybean isoflavones extracted from soybeanseed.The two-phase solvent system composed of ethyl acetate-acetic acid-water(5:l:10 in volume ratio).The mobile phase is thelower phase nd operated at flow-rate of 1.5ml/min, while the apparatus rotated at 800r/min.High performance liquidchromatographic analysis of Daidzin and Genistin revealed that their purity was individually 98.2%and 99.2%.

Key words: high speed countercurrent chromatography, separation, daidzin, genistin

JIANG He-Yuan, TAI Jian-Xiang, LU Fei-Jie . Separation of Daidzin and Genlstin from Soybean Isoflavones by High Speed Countercurrent Chromatography[J]. FOOD SCIENCE, 2004, 25(1): 85-88.

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Separation of Daidzin and Genlstin from Soybean Isoflavones by High Speed Countercurrent Chromatography

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