Species-specific PCR for Identification of Horse and Donkey Materials in Feedstuff

Abstract

Abstract: This study described a rapid PCR assay for the detection of bovine and sheep materials in functional food. Species-specific PCR primer had been used for this purpose and the enzymes DpnII and SspI were used to certify the PCR result for bovineand sheep respectively, the detection limitation was 0.05% for bovine derived material and 0.005% and 0.5% for goat and sheeprespectively. It could be one of an effective method for detection bovine and sheep derived materials and for verifying theauthenticity of the component of functional food.

Key words: functional food, bovine derived material, sheep derived material, PCR assay

CHEN Ying, QIAN Zeng-Min, XU Bao-Liang, WU Ya-Jun, WANG Jing, SU Ning. Species-specific PCR for Identification of Horse and Donkey Materials in Feedstuff[J]. FOOD SCIENCE, 2004, 25(10): 215-218.

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Species-specific PCR for Identification of Horse and Donkey Materials in Feedstuff

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