Studies on Screening by Mutating Strain of Producing Alpha-Transglucosidase and Its Conditions for Producing Alpha-Transglucosidase

Abstract

Abstract: Alpha-transglucosidase is a critical enzyme of producing isomaltooligosaccharide. In this paper, strain ASP-2 ofproducing α-transglucosidase was obtained by screening experiments. The optimum conditions for fermentation in solid-statewere:bran:water(m/m) =1:1,culture temperature and duration were 35℃ and 48h respectively, the optimum pH value was 6.0.Carbon sources and nitrogen sources were not necessary nutrition for producing enzyme in this culture medium , so they needn’tbe added in the culture medium in order to reduce production cost.

Key words: α-transglucosidase, strain, screening, conditions for producing α-transglucosidase

BI Jin-Feng, WEI Bao-Dong, LI Chang-Biao. Studies on Screening by Mutating Strain of Producing Alpha-Transglucosidase and Its Conditions for Producing Alpha-Transglucosidase[J]. FOOD SCIENCE, 2004, 25(10): 49-53.

[1]	ZHANG Boqin, FANG Zizhuang, CHENG Chifang, ZHANG Ruyi, DUAN Changqing, YAN Guoliang. Screening of Five Non-Saccharomyces Strains with Low Ethanol Yield and Evaluation of Their Oenological Properties [J]. FOOD SCIENCE, 2021, 42(8): 114-121.
[2]	WU Bohua, JIANG Xuewei, ZHANG Jinyu, RUAN Zhiqiang, LIANG Shengnan, CHEN Yongfa, ZHOU Shangting. Screening and Application of Bacillus spp. Promoting Moromi Fermentation [J]. FOOD SCIENCE, 2021, 42(6): 134-141.
[3]	GONG Min, MA Yanqiu, WANG Ruihong, CHI Yuan, CHI Yujie. Isolation and Identification of Typical Bacterial Strains from Eggshell Surface and Evaluation of Their Spoilage Potential in Liquid Whole Egg [J]. FOOD SCIENCE, 2021, 42(4): 153-160.
[4]	ZHU Xiaoling, LIU Jie, YU Tingting, ZHANG Li, WANG Huixia, WU Wanqin, CAO Qi, ZHANG Yazhen, YANG Zong. Rapid Screening of Pesticide Residues in Pepper Powder by High Performance Liquid Chromatography Coupled with Quadrupole-Time of Flight Mass Spectrometry [J]. FOOD SCIENCE, 2021, 42(4): 287-296.
[5]	CHEN Long, YANG Jun, MA Maomao, WU Shasha, YU Ping, ZENG Zheling. Strain Screening and Optimization of Fermentation Conditions for Protease Production Using Rapeseed Meal as a Nitrogen Source [J]. FOOD SCIENCE, 2021, 42(4): 115-121.
[6]	SUN Yue, LIU Jiayi, CHEN Lu, DU Hong, BAI Fengling, LÜ Xinran, ZHANG Defu, GUO Xiaohua, LI Jianrong. Screening of Lactic Acid Bacteria for Antagonistic Activity against Drug-Resistant Escherichia coli and Underlying Mechanism [J]. FOOD SCIENCE, 2021, 42(2): 121-127.
[7]	HAN Guoqiang, SUN Xieping, WU Pengfei, CHEN Jinzhao, CHEN Chun, WANG Qing. High Throughput Sequencing-based Analysis of Microbial Community Structure and Diversity during Baijiu Fermentation with Mixed-strain Xiaoqu [J]. FOOD SCIENCE, 2021, 42(18): 80-85.
[8]	LI Yijuan, SHAO Kaisheng, ZHANG Na, MENG Yingping, TAO Xueying, ZHOU Yingyu, WEI Hua, ZHANG Zhihong. Directional Screening for Reuterin-Producing Lactobacillus reuteri and Its Biological Characteristics [J]. FOOD SCIENCE, 2021, 42(14): 32-38.
[9]	WANG Chan, LI Rong, WU Yang, WANG Ying, TANG Shuhua, TAN Jin, JIANG Zitao. Rapid Screening of Radical Scavengers in Essential Oil from Piper longum L. as Food Seasoning [J]. FOOD SCIENCE, 2021, 42(14): 226-231.
[10]	DING Lili, LÜ Xinran, GAO Yongyue, CUI Xiaoling, WANG Xiaomi, BAI Fengling, YI Shumin, GUO Xiaohua. Screening for and Identification of Lactic Acid Bacteria with Antioxidant Activity from the Intestinal Tract of Fish [J]. FOOD SCIENCE, 2021, 42(10): 127-132.
[11]	ZHANG Yinghao, XUE Zhaoyang, ZHAO Tingbin, YIN Haisong, QIAO Changsheng,. Construction and Application of a Predictive Model for Determination of Polymalic Acid in the Fermentation Broth of Aureobasidium pullulans by Near-Infrared Spectroscopy [J]. FOOD SCIENCE, 2020, 41(8): 152-158.
[12]	LIU Shuxin, WU Aijuan, ZHEN Ni, SUN Jie, HUANG Ling, ZENG Zhidan, ZENG Xiaoqun, PAN Daodong. Screening for a Strain of Lactic Acid Bacteria with Broad-Spectrum Antimicrobial Activity and Analysis of Its Bacteriocin-Related Genes [J]. FOOD SCIENCE, 2020, 41(6): 101-107.
[13]	ZHANG Shixin, PENG Litao, FAN Ming, YANG Shuzhen, YAN Deng. Screening for Antifungal Agents of Clinical Drugs against Penicillium expansum on Postharvest Pear Fruit [J]. FOOD SCIENCE, 2020, 41(5): 180-185.
[14]	MENG Yong, WANG Jing, ZHU Xiaohua, GENG Xuebing, YANG Zong, SHEN Meifang. Screening and Confirmation of Multiple Antibiotic Residues in Aquatic Products by Triple Quadrupole-Linear Ion Trap Mass Spectrometry [J]. FOOD SCIENCE, 2020, 41(4): 313-318.
[15]	WU Lina, DONG Pengcheng, ZHANG Yimin, MAO Yanwei, LIANG Rongrong, YANG Xiaoyin, ZHU Lixian, LUO Xin. Biofilm Formation Ability of Escherichia coli O157:H7 on Stainless Steel Surface and Its Relationship with Bacterial Characteristics [J]. FOOD SCIENCE, 2020, 41(22): 127-132.

Studies on Screening by Mutating Strain of Producing Alpha-Transglucosidase and Its Conditions for Producing Alpha-Transglucosidase

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments