Abstract: A method for the determination on total flavanoids of Ampelopsis grossedentata was established: using 95% ethanolby continuous thermal reflux to extract its young shoots, taking dihydromyricetin as standard sample, selecting 294nm wave-length and by AlCl3 spectrometric method. The results showed that the regression equation, within the range of the standard sampleconcentration in 5-50μg/ml, was A=3.166×10-3X-9.0×10-3, with a good linearity(r=0.9999)between the absorbance andconcentration. The average recovery rate was 98.6% and RSD was 2.54% (n=5). The method is simple, rapid and accurate andsuitable for the quality evaluation of Ampelopsis grossedentata.

Key words: Ampelopsis grossedentata, total flavanoids, dihydromyricetin, spectrometric method