FOOD SCIENCE ›› 2004, Vol. 25 ›› Issue (4): 136-138.

Previous Articles     Next Articles

Extraction of Isoflavone Aglycones from Soybean Hypocotyl with C18 Column Chromatography

 QUAN  Ji-Shu, YIN  Xue-Zhe, GONG  Teng-Zhong-Guang   

  1. Department of Biochemistry, Yanbian University College of Medicine
  • Online:2004-04-15 Published:2011-10-24

Abstract: Total glycosides were extracted from soybean hypocotyl ,Where isoflavone glycosides were isolated with C18reversed phase column chromatography. Finally isoflavone aglycones were prepared by hydrolysis of isoflavone glycosides.The purity and contents of isoflavone aglycones were assayed with thin layer chromatograghy (TLC) and high-performance liquidchromatography (HPLC).

Key words: C18 column chromatography, Legumes, isoflavone aglycones