Abstract: In this article, the procedure of extracting rice bran oil from rice bran by aqueous enzymatic method was studied.Under the conditions of steam pretreatment, 0.5% of amylase, 0.2% of protease and extraction time 6h, the optimum technicalconditions of enzymatic extraction of rice bran oil were: extraction temperature 60℃, 1.2% of cellulase, pH 5.0 and the ratio ofsubstrate to solution as 1:5. Under these conditions, the yield of rice bran oil was 85.76%. Among the above factors, the quantityof the cellulase used was the most important one, and others in proper order as concentration of substrate, pH and temperatureas the least.

Key words: cellulase, aqueous enzymatic method, enzymatic treatment, rice bran oil