Application in Food Clenbuterol Poisoning of ELISA Screening and Confirmation by GC-MS

Abstract

Abstract: An enzyme-linked immunosorbant assay (ELISA) method and validation by GC-MS for clenbuterol residue in liver,kidney and muscle samples and biological samples was set up. The method was developed to enable rapid, specific, sensitivedetermination of clenbuterol residues in tissue samples and biological samples. Tissue samples were comminuted and extractedby perchloric acid solution, after liquid-liquid partition by mixture of isopropanol and ethyl acetate (40:60), the extracts wereapplied into the plate. Clenbuterol standard was spiked into liver and chicken samples by different levels respectively. Therecovery rate was from 50.5 to 92.0%, RSD was from 12.3 to 18.1%. Cooked toxic liver and blood and urine samples of toxicsubjects were determined. Positive samples from ELISA method were confirmed by gas chromatography-mass spectrometry.The ELISA results were consistent with the results of GC-MS.

Key words: ELISA, tissue samples and biological materials, clenbuterol

MIAO Hong, FENG Jin-Fang, ZHAO Jing-Ling, ZHAO Yun-Feng, WU Yong-Ning. Application in Food Clenbuterol Poisoning of ELISA Screening and Confirmation by GC-MS[J]. FOOD SCIENCE, 2004, 25(8): 160-163.

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Application in Food Clenbuterol Poisoning of ELISA Screening and Confirmation by GC-MS

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