Determination on Total Saponins of Momordica Charantia L. by Spectrophotometry

Abstract

Abstract: A method for the determination on total saponins of Momordica charantia L. was established: using MeOH by continuous thermal reflux to extract its total saponins, taking mordicoside A as standard sample, selecting 546nm wavelength and by spectrometric method. The results showed that the Total Saponins accounted for 0.0432% of the dried powder of Momordica charantia L. And the regression equation within the range of the standard sample concentration in 40～200μg was (A=0.0058C-0.1125) with a good linearity (R2=0.9994).The average recovery rate was 102.72% and RSD =3.99%.The method is simple, rapid and accurate and suitable for the quality evaluation of Momordica Charantia L. material and products.

Key words: Momordica charantia L., total saponins, Mordicoside A, spectrometric method, determination

XU Bin, DONG Ying. Determination on Total Saponins of Momordica Charantia L. by Spectrophotometry[J]. FOOD SCIENCE, 2005, 26(10): 165-169.

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Determination on Total Saponins of Momordica Charantia L. by Spectrophotometry

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